Control | 1 mM Thiopental | |
---|---|---|
Cerebellar neurons (from Qu et al., 2000) | ||
Medium | ||
[U-13C]Glu removed | 2443 ± 149 | 2460 ± 69 |
[U-13C]Lac | 66 ± 6 | 61 ± 4 |
Cell extract | ||
[1,2,3-13C]Glu | 62 ± 7 | 148 ± 91-a |
[1,2-13C]Glu | 20 ± 4 | 46 ± 31-a |
[U-13C]Asp | 36 ± 6 | 97 ± 191-a |
Cerebellar astrocytes (from Qu et al., 2001) | ||
Medium | ||
[U-13C]Glu removed | 2075 ± 58 | 2075 ± 105 |
[U-13C]Lac | 212 ± 12 | 171 ± 91-a |
[U-13C]Gln | 265 ± 16 | 255 ± 11 |
Cell Extract | ||
[1,2,3-13C]Glu | 61 ± 5 | 77 ± 51-a |
[U-13C]Gln | 24 ± 3 | 39 ± 41-a |
[U-13C]Asp | 53 ± 6 | 55 ± 4 |
Cortical astrocytes (from Qu et al., 1999) | ||
Medium | ||
[U-13C]Glu removed | 2272 ± 72 | 1028 ± 321-a |
[U-13C]Lac | 178 ± 19 | 78 ± 41-a |
[U-13C]Gln | 307 ± 12 | 196 ± 61-a |
Cell extract | ||
[1,2,3-13C]Glu | 188 ± 16 | 144 ± 31-a |
[U-13C]Gln | 49 ± 4 | 47.7 ± 1 |
[U-13C]Asp | 53 ± 6 | 30 ± 11-a |
All cultures were incubated with [U-13C]glutamate (0.5 mM) and 0 or 1 mM sodium thiopental for 2 h. The C-3 resonance was used for 13C MR determination except for lactate where the C-2 resonance was used. Superscripts indicate statistical differences as determined by analysis of variance followed by post hoc test for multiple comparisons (p < 0.05 was considered significant).
↵1-a Significantly different from controls.