Table 1

Content of ¹³C (nmol/mg protein) in metabolites from lyophilized cell extracts and medium of cerebellar neurons and cerebellar and cortical astrocytes after incubation with [U-¹³C]glutamate for 2 h in the presence and absence of thiopental

	Control	1 mM Thiopental
Cerebellar neurons (from Qu et al., 2000)
Medium
[U-¹³C]Glu removed	2443 ± 149	2460 ± 69
[U-¹³C]Lac	66 ± 6	61 ± 4
Cell extract
[1,2,3-¹³C]Glu	62 ± 7	148 ± 9^1-a
[1,2-¹³C]Glu	20 ± 4	46 ± 3^1-a
[U-¹³C]Asp	36 ± 6	97 ± 19^1-a
Cerebellar astrocytes (from Qu et al., 2001)
Medium
[U-¹³C]Glu removed	2075 ± 58	2075 ± 105
[U-¹³C]Lac	212 ± 12	171 ± 9^1-a
[U-¹³C]Gln	265 ± 16	255 ± 11
Cell Extract
[1,2,3-¹³C]Glu	61 ± 5	77 ± 5^1-a
[U-¹³C]Gln	24 ± 3	39 ± 4^1-a
[U-¹³C]Asp	53 ± 6	55 ± 4
Cortical astrocytes (from Qu et al., 1999)
Medium
[U-¹³C]Glu removed	2272 ± 72	1028 ± 32^1-a
[U-¹³C]Lac	178 ± 19	78 ± 4^1-a
[U-¹³C]Gln	307 ± 12	196 ± 6^1-a
Cell extract
[1,2,3-¹³C]Glu	188 ± 16	144 ± 3^1-a
[U-¹³C]Gln	49 ± 4	47.7 ± 1
[U-¹³C]Asp	53 ± 6	30 ± 1^1-a

All cultures were incubated with [U-¹³C]glutamate (0.5 mM) and 0 or 1 mM sodium thiopental for 2 h. The C-3 resonance was used for ¹³C MR determination except for lactate where the C-2 resonance was used. Superscripts indicate statistical differences as determined by analysis of variance followed by post hoc test for multiple comparisons (p < 0.05 was considered significant).

↵1-a Significantly different from controls.