Distribution of β-galactosidase activity in the organs of newborn pigs 30 min after injection of the conjugates into the right coronary artery
| Organ | Control Baseline | Conjugate | (Anti-PECAM-Base)/(IgG-Base) | |
|---|---|---|---|---|
| Anti-PECAM/SA-β-Gal | IgG/SA-β-Gal | |||
| Lung | 1.2 ± 0.1 | 27.7 ± 5.63-150 | 2.2 ± 0.3 | 26.3 |
| Liver | 4.0 ± 0.4 | 12.5 ± 4.0 | 5.8 ± 0.4 | 4.8 |
| Spleen | 6.0 ± 1.1 | 9.7 ± 0.7 | 7.8 ± 1.3 | 2.0 |
| Kidney | 1.2 ± 0.2 | 4.6 ± 0.3 | 3.5 ± 0.9 | 1.1 |
| Heart3-150 | 1.3 ± 0.1 | 5.5 ± 1.33-150 | 2.1 ± 0.3 | 5.2 |
SA-β-Gal (1 mg) conjugated either to anti-PECAM or IgG was injected into the right coronary artery of intact newborn pigs. A control group included intact pigs (n = 3). Thirty minutes after injection of the conjugate, the pigs were desanguinated and perfused through a femoral artery catheter with 1 liter of sterile saline to remove blood of the internal tissues. The organs were harvested, flash frozen, and homogenized. Results of the value of the β-Gal activity in the tissue homogenate are expressed in milliunits of β-Gal activity per milligram of protein (mean ± S.E.M.,n = 3). In the lungs and heart (the right atrium is shown), the activity was significantly higher after injection of anti-PECAM/SA-β-Gal than in the IgG/SA-β-Gal group (
↵3-150 p < 0.001).