Parameter Measured | Male Donors | Female Donors | P1-a |
---|---|---|---|
Number of the donors | 21 | 12 | |
Age of the donors (yr) | 47 ± 3 | 56 ± 16 | |
Adjusted protein content (mg/ml) | 20 | 20 | |
Total P450 content1-b | 0.45 ± 0.29 | 0.43 ± 0.20 | 0.851 |
Cytochrome b 5content1-c | 0.46 ± 0.12 | 0.38 ± 0.13 | 0.085 |
NADPH-cytochrome creductase1-c | 205.3 ± 63.2 | 195.9 ± 53.3 | 0.667 |
Caffeine N3-demethylation (1A2)1-d | 47.0 ± 24.2 | 33.1 ± 50.5 | 0.291 |
Coumarin 7-hydroxylation (2A6)1-d | 1769.8 ± 1878.9 | 1470.6 ± 1283.3 | 0.630 |
S-Mephenytoin N-demethylation (2B6)1-d | 136.6 ± 210.9 | 107.6 ± 111.6 | 0.662 |
Tolbutamide methyl-hydroxylation (2C9)1-d | 291.7 ± 216.1 | 213.4 ± 86.0 | 0.241 |
S-Mephenytoin 4′-hydroxylation (2C19)1-d | 73.7 ± 130.3 | 93.4 ± 133.2 | 0.681 |
Dextromethorphan O-demethylation (2D6)1-d | 255.0 ± 171.8 | 125.3 ± 97.0 | 0.023 |
Chlorzoxazone 6-hydroxylation (2E1)1-d | 2202.1 ± 601.3 | 1648.0 ± 846.9 | 0.036 |
Dextromethorphan N-demethylation (3A4)1-d | 115.1 ± 194.9 | 109.2 ± 101.9 | 0.923 |
Testosterone 6β-hydroxylation (3A4/5)1-d | 3963.5 ± 4951.6 | 4981.2 ± 3944.7 | 0.547 |
Lauric acid 12-hydroxylation (4A11)1-d | 1145.2 ± 524.1 | 927.4 ± 477.1 | 0.245 |
Note: The contents of total P450, b5, and cytochrome c reductase and the activities for various P450 isoforms were determined according the methods described (Pearce et al. (1996).
↵1-a P values were calculated by using the two-tailed, unpaired t test.
↵1-b Total P450 content is expressed as nanomoles per milligram of protein.
↵1-c Cytochrome b5 content is expressed as nanomoles per milligram of protein per minute.
↵1-d P450 dependent activities are expressed as picomoles per milligram of protein per minute.