Km and Ki values of PAH and pravastatin uptake by kidney slices, rOat1 and rOat3.
| PAH Uptake | Pravastatin Uptake | |||
|---|---|---|---|---|
| Oat1-LLC | Kidney Slice | Oat3-LLC | Kidney Slice | |
| PAH | K m (μM) = 59.9 ± 5.0 | K m (μM) = 69.0 ± 8.6 | K i (μM) = 1350 ± 510 | K i (μM) = 2090 ± 430 |
| pravastatin | K i (μM) = 1150 ± 460 | K i (μM) = 937 ± 402 | K m (μM) = 13.4 ± 2.0 | K m (μM) = 11.4 ± 3.1 |
| PCG | K i (μM) = 418 ± 42 | K i (μM) = 1930 ± 330 | K i (μM) = 52.8 ± 9.0 | K i (μM) = 94.0 ± 44.2 |
| DBSP | K i (μM) = 2.74 ± 0.26 | K i (μM) = 22.2 ± 5.8 | K i (μM) = 3.09 ± 0.63 | K i (μM) = 17.0 ± 4.4 |
Data shown in Figs. 3, 4, and 5 were used to determine theKm and Ki values for the uptake of PAH and pravastatin by cDNA-transfected cells and kidney slices. The Km and Kivalues were determined by nonlinear regression analysis.