Table 1

Km and Ki values of PAH and pravastatin uptake by kidney slices, rOat1 and rOat3.

PAH UptakePravastatin Uptake
Oat1-LLCKidney SliceOat3-LLCKidney Slice
PAH K m (μM)  =  59.9  ± 5.0 K m (μM)  =  69.0  ± 8.6 K i (μM)  =  1350  ± 510 K i (μM)  =  2090  ± 430
pravastatin K i (μM)  =  1150  ± 460 K i (μM)  =  937  ± 402 K m (μM)  =  13.4  ± 2.0 K m (μM)  =  11.4  ± 3.1
PCG K i (μM)  =  418  ± 42 K i (μM)  =  1930  ± 330 K i (μM)  =  52.8  ± 9.0 K i (μM)  =  94.0  ± 44.2
DBSP K i (μM)  =  2.74  ± 0.26 K i (μM)  =  22.2  ± 5.8 K i (μM)  =  3.09  ± 0.63 K i (μM)  =  17.0  ± 4.4

Data shown in Figs. 3, 4, and 5 were used to determine theKm and Ki values for the uptake of PAH and pravastatin by cDNA-transfected cells and kidney slices. The Km and Kivalues were determined by nonlinear regression analysis.