Antagonist binding properties of wild-type and mutant receptors
| Subtype | N-Methylscopolamine Kd(± S.E.M.) |
|---|---|
| nM | |
| M1 | 0.018 ± 0.006 (3) |
| M1-YP | 0.020 ± 0.007 (3) |
| M2 | 0.0440 ± 0.0005 (2) |
| M2-YP | 0.056 ± 0.002 (2) |
| M3 | 0.0180 ± 0.0025 (2) |
| M3-YP | 0.0190 ± 0.0005 (2) |
| M4 | 0.021 ± 0.005 (3) |
| M4-YP | 0.021 ± 0.005 (3) |
| M5 | 0.020 ± 0.002 (3) |
| M5-YP | 0.019 ± 0.003 (3) |
Assays were performed using membranes derived from transfected COS-7 cells. Data represent the average (± S.E.M) of the number of experiments indicated in parentheses.