Effects of combinations of meAEA and capsaicin upon the proliferation of C6 glioma cells
| Capsaicin | Antagonists | Cell Number (× 103 well−1) after [meAEA] (μM) of: | ||
|---|---|---|---|---|
| 0 | 1 | 10 | ||
| μM | ||||
| 0 | − | 30.9 ± 0.70 | 28.0 ± 3.2 | 13.7 ± 3.7 |
| 0 | + | 30.4 ± 3.9 | 26.0 ± 3.0 | 7.3 ± 1.9 |
| 1 | − | 29.7 ± 3.4 | 27.1 ± 1.6 | 8.1 ± 1.8 |
| 1 | + | 26.8 ± 3.2 | 23.4 ± 2.9 | 4.2 ± 0.67 |
| 10 | − | 23.0 ± 2.8 | 24.1 ± 1.0 | 4.8 ± 1.1 |
| 10 | + | 23.6 ± 3.3 | 19.9 ± 2.9 | 2.4 ± 0.43 |
C6 glioma cells were treated daily for 4 days with the concentrations of compounds shown, as described under Experimental Procedures. Shown are means ± S.E.M., n = 4. In the absence of any solvents, the cell number was 33.4 ± 2.9 × 103 well−1. In the “Antagonists” column, − and + refer to solvent control and the combination of AM251 (0.3 μM), AM630 (0.3 μM), and capsazepine (1 μM), respectively. Two-way ANOVA of the results obtained in the absence of antagonists revealed a significant effect of both capsaicin (F2,27 = 6.26; p = 0.006) and meAEA (F2,27 = 57.47; p < 0.0001) but no interaction between the compounds (F4,27 = 0.53;p = 0.72).