Comparison of methods used to measure rifampin induction in human hepatocytes
| A. Gene Name/Description | Oligo | RNase | cDNA | B. Housekeeping Genes | Oligo | cDNA |
|---|---|---|---|---|---|---|
| Cytochrome P450 1A2 | 0.4 | 2.6 | 2.2 | Ubiquitin | 0.9 | 0.9 |
| Cytochrome P450 2C8 | 6.5 | *3.0 | Phospholipase A2 | 0.7 | 0.8 | |
| Cytochrome P450 2C9 | 3.7 | 4.4 | GAPDH | 1.0 | 1.0 | |
| Cytochrome P450 2C18 | 0.6 | α-Tubulin | 0.8 | 0.7 | ||
| Cytochrome P450 2D6 | N.D. | 1.3 | 1.3 | 40S ribosomal protein | 1.0 | 1.0 |
| Cytochrome P450 2E1 | 1.0 | 1.8 | 1.8 | |||
| Cytochrome P450 3A4 | 55.1 | 62.7 |
Tables comparing the -fold induction as determined by separate methods of detection on the same RNA sample. (A) -Fold induction of cytochrome P450 enzymes (*, cDNA-based microarrays were not able to discriminate between cytochrome P450 2C family members) and (B) -fold induction of housekeeping genes using microarrays. N.D., not determined.