Table 3

Comparison of methods used to measure rifampin induction in human hepatocytes

A. Gene Name/DescriptionOligoRNasecDNAB. Housekeeping GenesOligocDNA
 Cytochrome P450 1A20.42.62.2 Ubiquitin0.90.9
 Cytochrome P450 2C86.5 *3.0 Phospholipase A20.70.8
 Cytochrome P450 2C93.74.4 GAPDH1.01.0
 Cytochrome P450 2C180.6 α-Tubulin0.80.7
 Cytochrome P450 2D6N.D.1.31.3 40S ribosomal protein1.01.0
 Cytochrome P450 2E11.01.81.8
 Cytochrome P450 3A455.162.7

Tables comparing the -fold induction as determined by separate methods of detection on the same RNA sample. (A) -Fold induction of cytochrome P450 enzymes (*, cDNA-based microarrays were not able to discriminate between cytochrome P450 2C family members) and (B) -fold induction of housekeeping genes using microarrays. N.D., not determined.