Regioselective composition of HETEs produced by CYP2C recombinant proteins
| Regioisomer | Distribution | ||||
|---|---|---|---|---|---|
| CYP2C37 | CYP2C38 | CYP2C39 | CYP2C40 | CYP2C29 | |
| % | |||||
| 5-HETE | N.D. | 1 | 2 | N.D. | N.D. |
| 8-HETE | 19 | 8 | 6 | N.D. | N.D. |
| 9-HETE | 4 | 2 | N.D. | N.D. | N.D. |
| 11-HETE | 11 | 8 | 38 | N.D. | N.D. |
| 12-HETE | 12 | 24 | 17 | N.D. | N.D. |
| 15-HETE | 2 | 6 | 29 | N.D. | N.D. |
| 16-HETE | N.D. | 2 | 5 | 95 | N.D. |
| 17-HETE | 2 | 1 | N.D. | N.D. | N.D. |
| 18-HETE | 12 | 15 | 3 | N.D. | N.D. |
| 19-HETE | 10 | 11 | N.D. | N.D. | N.D. |
| 20-HETE | 15 | 5 | N.D. | 5 | N.D. |
| Bisallylic HETEs | 13 | 17 | N.D. | N.D. | N.D. |
The regiochemical distribution of HETEs formed by incubation of murine CYP2C proteins with [1-14C]AA was quantified by HPLC and liquid scintillation as described under Experimental Procedures.
N.D., nondetectable.