Gene-specific primers and probes for TaqMan analysis
| Gene2-a | Forward primer2-b | Probe2-b | Reverse primer2-b | Base Pair2-c |
|---|---|---|---|---|
| MDR1 (ABCB1) | CAGACAGCAGGAAATGAAGTTGAA | TTTCACTTTTGGATTCATCAGCTGCATTTTCTA | TGAAGACATTTCCAAGGCATCA | 2001–2081 |
| MDR3 (ABCB4) | TCAGGAAGCCAGATCCAGTCA | AGTGGCAGCCTTTTCATCATTTAGTTCAAATTCTT | CGAGATTTCCAGCCATTTGG | 1929–2014 |
| ABCB5 | GACATCGGCTGGTTTGATAGCT | TGACATCGGTGAACTTAACACTCGCAT | GAGTTTCCAGCCCTTCACCA | 57103–57355 |
| MRP1 (ABCC1) | GAAGGCCATCGGACTCTTCA | CTCCTTCCTCAGCATCTTCCTTTTCATGTG | CAGCGCGGACACATGGT | 3097–3166 |
| MRP2 (ABCC2) | TGCAGCCTCCATAACCATGAG | AGAGAGAACAGCTTTCGTCGAACACTTAGCC | GATGCCTGCCATTGGACCTA | 2728–2807 |
| MRP3 (ABCC3) | CACACGGATCTGACAGACAATGA | CCAGTCACCTATGTGGTCCAGAAGCAGTTT | ACAGGGCACTCAGCTGTCTCA | 2670–2745 |
| MRP4 (ABCC4) | AAGTGAACAACCTCCAGTTCCAG | CAAACCGAAGACTCTGAGAAGGTACGATTCCT | GGCTCTCCAGAGCACCATCT | 2026–2144 |
| MRP5 (ABCC5) | TGAAAGCCATTCGAGGAGTTG | CTCGCAGCGTGCCCTTGACAAAG | CGGAAAAGCTCGTCATGCA | 2979–3054 |
| MRP6 (ABCC6) | AGACACGGTTGACGTGGACAT | CCAGACAAACTCCGGTCCCTGCTGAT | GCTGACCTCCAGGAGTCCAA | 3156–3231 |
| BCRP (ABCG2) | CAGGTCTGTTGGTCAATCTCACA | CCATTGCATCTTGGCTGTCATGGCTT | TCCATATCGTGGAATGCTGAAG | 1893–1969 |
| LRP (MVP) | GGCCTCTGTCACTTTCGATGA | TTCCATAAGAACTCAGCCCGCATCATTC | CTTCCGAGGTCTCAAAGCCA | 1854–1936 |
| CYP3A4 | AGAAAGTCGCCTCGAAGATACAC | TCTGGAGCTCGTGGCCCAATCAATTATC | TGCAGTTTCTGCTGGACATC | 780–1004 |
| Villin 1 (VIL1) | CATGAGCCATGCGCTGAAC | TCATCAAAGCCAAGCAGTACCCACCAAG | TCATTCTGCACCTCCACCTGT | 957–1028 |
↵2-a Name within parentheses or single name corresponds to the Homo sapiens Official Gene Symbol, according to the HUGO Gene Nomenclature Committee (http://www.gene.ucl.ac.uk/nomenclature/).
↵2-b Sequences of the primers and the probe are written in the 5′ to 3′ direction.
↵2-c Area amplified refers to sequences deposited at GenBank with accession numbers as follows: AF016535(MDR1), M23234 (MDR3), AC002486(ABCB5), L05628 (MRP1), U63970 (MRP2),AB010887 (MRP3), AF071202 (MRP4), AF146074(MRP5), AF168791 (MRP6), AF098951(BCRP), X79882 (LRP), AF182273(CYP3A4), and X12901 (Villin).