Effect of ion substitution on initial uptake rate of [14C]pravastatin (10 μM), [3H]estradiol-17β-d-glucuronide (10 μM), and [3H]taurocholic acid (10 μM) by human hepatocytes
| Uptake Rate | Na+-Independent Ratio Choline+/Na+ | ||
|---|---|---|---|
| Na+ | Choline+ | ||
| pmol/min/106 cells | |||
| Pravastatin | 10.5 ± 4.3 | 13.5 ± 6.1 | 1.29 |
| E2-17G | 31.3 ± 8.4 | 39.5 ± 14.4 | 1.26 |
| TCA | 23.7 ± 3.1 | 4.7 ± 0.6 | 0.20 |
The initial uptake rate of each compound by human hepatocytes was determined in Na+-containing buffer or choline+-containing buffer (see Experimental Procedures). Data are expressed as the mean ± S.E. of three uptake measurements.
E2-17G, estradiol-17β-d-glucuronide; TCA, taurocholic acid.