SUPERFIT Pretreatment | Kd | Bmax | |
---|---|---|---|
nM | pmol/mg | % control | |
0 | 1.2 ± .10 | 2.63 ± .14 | 100 |
0.6 | 2.6 ± .72 | 2.01 ± .47 | 76 |
6 | 2.1 ± .18 | 1.19 ± .07 | 45 |
12 | 1.0 ± .04 | .50 ± .04 | 19 |
60 | 2.3 ± .60 | .051 ± .015 | 2 |
GH3MORDOR cells were incubated with the indicated concentration of SUPERFIT for 45 min, washed extensively, and membranes were prepared. Receptor density (Bmax) and affinity (Kd) values were determined from Scatchard analysis of saturation binding utilizing [3H]DPDPE as described underExperimental Procedures. The Kd andBmax values represent estimates for each parameter ±S.E.M. determined by the computer program LIGAND from a single experiment performed in triplicate.