Table 1

Binding parameters for cannabinoid-stimulated [35S]GTPγS binding and [3H]SR141716A binding in brain membranes

RegionNet Levonantradol-Stimulated [35S]GTPγS BindingSpecific [3H]SR141716A Binding (Bmax)
pmol/mg % stimulation pmol/mg
Cerebellum0.24  ± 0.02620  ± 363.9  ± 0.5(5.9)
Hippocampus0.21  ± 0.01210  ± 123.7  ± 0.2(5.5)
Hypothalamus0.25  ± 0.01140  ± 151.5  ± 0.3(2.3)

Membranes from the indicated regions were analyzed for maximal stimulation of [35S]GTPγS binding by 3 μM levonantradol and for [3H]SR141716A binding. Data for [35S]GTPγS binding are presented as net levonantradol-stimulated binding (pmol/mg), and percentage of stimulation of [35S]GTPγS binding over basal by levonantradol. Data for [3H]SR141716A binding are presented as specific binding determined with 0.5 nM [3H]SR141716A, and the estimated Bmax of [3H]SR141716A binding assuming a KD of 0.24 nM for SR141716A. Data are mean values ± S.E. from four separate experiments.