Table 1

Effect of NSAIDs on hOAT1-mediated cytotoxicity of adefovir

InhibitorAdefovir 50% Cytotoxic Conc.
CHOpIRESCHOhOATCHOpIRES/CHOhOA
μM
−275  ± 250.7  ± 0.2393
Probenecid280  ± 673.7  ± 0.9 (5.3)1-a 75
Diclofenac300  ± 03.6  ± 2.4 (5.2)83
Diflunisal1-b 200  ± 02  ± 0 (2.9)100
Flurbiprofen65  ± 55.5  ± 0.5 (7.9)12
Ibuprofen130  ± 474.8  ± 0.2 (6.9)27
Indomethacin55  ± 01.2  ± 0 (1.7)46
Ketoprofen75  ± 1211.4  ± 2.8 (16.2)7
Naproxen83  ± 117  ± 1.3 (10)12
Piroxicam310  ± 901.35  ± 0.65 (1.9)221

CHOpIRES and CHOhOAT cells were treated with various concentrations of adefovir in the presence or absence of 100 μM inhibitor. After 5 days, 50% cytotoxic concentrations values were determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide cytotoxicity assay. The data represent mean ± S.D. from at least two independent experiments.

  • ↵1-a  The numbers in parentheses represent fold decrease of adefovir cytotoxicity in CHOhOAT in the presence of inhibitor.

  • ↵1-b  Diflunisal was tested at 30 μM concentration due to its cytotoxicity at higher concentrations.