Table 1

Receptor binding affinities for SB-277011-A in radioligand binding assays

ReceptorpKiReceptorpKi
hD3 7.95  ± 0.02rD3 7.97  ± 0.04
hD2 5.98  ± 0.04rD2 5.55  ± 0.05
h5-HT1A <5.3h5-HT2A <5
h5-HT1B <5.2h5-HT2B 5.89  ± 0.09
h5-HT1D 5.79  ± 0.06h5-HT2C <5
h5-HT1E <5 g-p5-HT4 <5.3
h5-HT1F <5.2h5-HT6 <5
h5-HT7 <5

Data are mean ± S.E. from at least three independent determinations using cell lines stably expressing cloned receptors. Receptors and radioligands were hD2, hD3 (human cloned receptors in CHO cells; [125I]iodosulpride); rD2, rD3 (rat cloned receptors in CHO cells; [125I]iodosulpride); h5-HT1A (human cloned receptors in HEK 293 cells; [3H]8-OH-DPAT); h5-HT1B (human cloned receptors in CHO cells; [3H]5-HT); h5-HT1D(human cloned receptors in CHO cells; [3H]5-HT); h5-HT1E (human cloned receptors in CHO cells; [3H]5-HT); h5-HT1F (human cloned receptors in CHO cells; [3H]5-HT); h5-HT2A (human cloned receptors in HEK 293 cells; [3H]ketanserin); h5-HT2B (human cloned receptors in HEK 293 cells; [3H]5-HT); h5-HT2C (human cloned receptors in HEK 293 cells; [3H]mesulergine); gp-5-HT4 (guinea pig hippocampal membranes); h5-HT6(human cloned receptors in HeLa cells; [3H]LSD); h5-HT7 (human cloned receptors in HEK 293 cells; [3H]5-carboxamidotryptamine).