pIC50 values (M) for peptides in competition for binding of125I-hCT to COS-7 cells transfected with hCTRl1− (100 ng) and RAMP or vector DNA (150 ng)
| sCT | Sites | hCT | Sites | Rat Amylin | Sites | hCGRPα | Sites | |
|---|---|---|---|---|---|---|---|---|
| % | % | % | % | |||||
| pcDNA3 | ||||||||
| Site 1 | 11.20 ± 0.31 | 35.8 ± 11.5 | 9.89 ± 0.25 | 54.7 ± 12.8 | ||||
| Site 2 | 9.57 ± 0.18 | 64.2 | 8.02 ± 0.31 | 45.3 | 6.27 ± 0.13 | 100 | 5.68 ± 0.13 | 100 |
| RAMP1 | ||||||||
| Site 1 | 11.07 ± 0.21 | 74.3 ± 17.2 | 9.90 ± 0.18 | 53.7 ± 7.8 | ||||
| Site 2 | 9.50 ± 0.73 | 25.7 | 7.98 ± 0.21 | 46.3 | 6.30 ± 0.11 | 100 | 5.74 ± 0.14 | 100 |
| RAMP2 | ||||||||
| Site 1 | 11.12 ± 0.38 | 54.6 ± 21.8 | 10.68 ± 0.21 | 44.2 ± 5.7 | 9.34 ± 0.53 | 21.6 ± 5.5 | ||
| Site 2 | 9.48 ± 0.53 | 45.4 | 8.22 ± 0.17 | 55.8 | 6.23 ± 0.15 | 78.4 | 5.84 ± 0.10 | 100 |
| RAMP3 | ||||||||
| Site 1 | 11.11 ± 0.32 | 53.9 ± 17.5 | 10.78 ± 0.31 | 35.9 ± 6.9 | 8.61 ± 0.72 | 20.0 ± 8.5 | ||
| Site 2 | 9.45 ± 0.41 | 46.1 | 8.30 ± 0.18 | 64.1 | 6.13 ± 0.18 | 80 | 5.74 ± 0.12 | 100 |
Data are presented as mean ± S.E. (n = 3).