pIC50 values (M) for peptides in competition for binding of125I-hCT to COS-7 cells transfected with hCTRl1+ (100 ng) and RAMP or vector DNA (150 ng)
| sCT | Sites | hCT | Sites | Rat Amylin | Sites | hCGRPα | Sites | |
|---|---|---|---|---|---|---|---|---|
| % | % | % | % | |||||
| pcDNA3 | ||||||||
| Site 1 | 9.91 ± 0.07 | 100 | 10.53 ± 0.33 | 37.8 ± 7.3 | 10.76 ± 0.6 | 19.4 ± 4.3 | 9.23 ± 0.71 | 13.6 ± 4.4 |
| Site 2 | 8.31 ± 0.16 | 62.2 | 6.38 ± 0.15 | 80.6 | 5.86 ± 0.12 | 86.4 | ||
| RAMP1 | ||||||||
| Site 1 | 10.11 ± 0.06 | 100 | 10.02 ± 0.25 | 46 ± 9.3 | 9.78 ± 0.57 | 23.5 ± 5.7 | 9.99 ± 0.5 | 18.7 ± 3.6 |
| Site 2 | 8.07 ± 0.21 | 54 | 6.12 ± 0.18 | 76.5 | 5.76 ± 0.14 | 81.3 | ||
| RAMP2 | ||||||||
| Site 1 | 4-1509.76 ± 0.05 | 100 | 10.92 ± 0.21 | 28.6 ± 3.3 | 10.00 ± 0.32 | 29.3 ± 4.2 | 8.62 ± 0.36 | 28.3 ± 5.7 |
| Site 2 | 8.19 ± 0.08 | 71.4 | 6.43 ± 0.14 | 70.7 | 5.98 ± 0.14 | 71.7 | ||
| RAMP3 | ||||||||
| Site 1 | 10.05 ± 0.05 | 100 | 10.72 ± 0.16 | 34.3 ± 3.6 | 8.92 ± 0.38 | 30.6 ± 6.6 | 9.61 ± 0.68 | 19.2 ± 5.4 |
| Site 2 | 8.37 ± 0.09 | 65.7 | 6.17 ± 0.18 | 69.4 | 5.9 ± 0.17 | 80.8 |
Data are presented as mean ± S.E. (n = 4).
↵4-150 P < .05 versus R1, R3.