Table 1

Effect of naloxone on high-affinity [3H]dopaminergic uptake by midbrain neurons after LPS treatment

Treatment TimeLPSDopamine Uptake
LPS Alone1 μM Nal + LPS
h ng/ml % control
90100.0  ± 3.0
0.195.6  ± 20.2
195.0  ± 12.7
1096.0  ± 11.3
10086.3  ± 19.9
160100.0  ± 10.893.6  ± 17.4
0.186.5  ± 7.793.5  ± 4.1
167.8  ± 10.81-150 96.0  ± 8.5
1058.1  ± 14.4** 88.6  ± 5.4
10050.1  ± 5.1** 85.0  ± 4.8
240102.6  ± 4.9114.8  ± 5.5
0.164.8  ± 8.1** 92.3  ± 5.4
145.6  ± 7.8** 80.5  ± 7.8
1043.3  ± 5.7** 75.4  ± 9.3
10035.6  ± 5.1** 68.5  ± 4.0
480100.5  ± 5.2104.3  ± 3.5
0.166.7  ± 8.31-150 90.0  ± 3.9
141.4  ± 9.7** 81.4  ± 0.3
1034.5  ± 12.5** 63.7  ± 5.1
10031.1  ± 10.6** 65.0  ± 8.6

The results are expressed as the percentage of the control uptake of dopaminergic and are the mean ± S.E. of three or four experiments performed in triplicate. The value for control group was 449.6 ± 67.4 fmol of dopaminergic/well.

  • Nal, naloxone.

  • 1-150   P < .05 and **  P < .005, compared with the untreated control cultures, respectively;   P < .05 and P < .005, compared with the corresponding LPS-treated cultures, respectively.