Matrix | Protein Concentration | Free Fraction |
---|---|---|
μg/ml | ||
Pooled human liver microsomes | 50 | 1.075 |
100 | 0.878 | |
200 | 0.629 | |
500 | 0.324 | |
Pooled human liver microsomes (heat-inactivated) | 200 | 0.502 |
Lymphoblast microsomes | 250 | 0.816 |
500 | 0.55 | |
1000 | 0.257 | |
Insect cell microsomes | 250 | 0.675 |
Buffer (control) | – | 1.192 |
Values are unbound fractions determined at a spiked drug concentration of 50 μg/ml.