Treatment3-a | Number of Animals Tested | Number of Animals with Keratinization Grade | ||
---|---|---|---|---|
0 | + | ++ | ||
Vehicle | 6 | 0 | 0 | 6 |
R115866 (5 mg/kg) | 6 | 6 | 0 | 0 |
RA (20 mg/kg) | 6 | 6 | 0 | 0 |
R115866 (5 mg/kg) + AGN193109 (5 mg/kg) | 6 | 0 | 4 | 2 |
RA (20 mg/kg) + AGN193109 (5 mg/kg) | 6 | 0 | 4 | 2 |
AGN193109 (5 mg/kg) | 6 | 0 | 0 | 6 |
↵3-a Vehicle (PEG 200), R115866, RA, and AGN193109 were administered p.o., either alone or combined as specified, once daily for 3 days to estrogen-stimulated ovariectomized rats. One day after the third administration, the intensity of the vaginal keratinization was assessed as described in Materials and Methods.