Table 2

Binding affinity of PEA in a variety of membrane preparations

Tissue% Displacement of Bound [3H]CP55,940 (at 100 μM)
Cerebellum (with PMSF)23.2
Cerebellum (without PMSF)19.2
Human CB2-293 cells50.2
Rat CB2-293 cells46.2

Inhibition constants were obtained from experiments using stably transfected 293 cells or from rat cerebellar membranes (seeExperimental Procedures).