Table 4

Immunohistological characterization of adhesion molecules and chemokines responsible for mononuclear cell accumulation in tissues after phosphorothioate oligonucleotide treatment in mice

Saline ControlISIS 1082ISIS 13108ISIS 5132ISIS 13650
LiverKidneyLiverKidneyLiverKidneyLiverKidneyLiverKidney
Adhesion molecules
 LFA-1 (CD11a)3+3+2+1+
 Mac-1 (CD11b)3+3+2+3+
 VLA-1 α (CD49)2+2+2+1+neg1+1+
 T cells (CD4)3+
 Ly6G2+
 HPCA (CD34)2+2+3+4+2+2+2+2+2+2+
 ICAM-1 (CD54)2+wk2+2+NDND2+2+2+2+
 VCAM (CD106)wkwk2+3+NDND1+wk
 LFA-β3+3+NDND2+2+2+
 PECAM (CD31)2+1+3+3+2+2+2+1+1+3+
 B7-1 (CD80)
 B7-2 (CD86)
 MadCAM
Cytokines
 TNF Alpha0000NDND0000
 IL-10wkwk2+2+NDNDNDNDNDND
 IL-6wk1+2+2+wkwkwkwkwkwk
  • 0, no staining; wk, weak; 1+, minimal; 2+, moderate; 3+, heavy; ND, not determined; HPCA, hematopoietic progenitor cell adhesion; PECAM, platelet/endothelial cell adhesion molecule; MadCAM, mucosal addressin cell adhesion molecule.