Relative expression of cMOAT/MRP2 and MRP3 in Caco-2 cells at various culture periods
| Culture Days after Confluence | Relative Expression | |
|---|---|---|
| cMOAT/MRP2 | MRP3 | |
| 0 | 1.00 | 1.00 |
| 5 | 2.01 | 5.79 |
| 7 | 1.87 | 6.18 |
| 10 | 1.82 | 10.74 |
| 16 | 1.18 | 4.77 |
Caco-2 cells were cultured for 0, 5, 7, 10, and 16 days after confluence was reached. The expression level of cMOAT/MRP2, MRP3, and GAPDH was quantified by analyzing the results of Northern blot (Fig. 4) with a densitometer. The relative expression was determined by dividing the expression levels of transporters by those of GAPDH.