Table 1

Comparison of binding affinities of cannabinoid ligands to CB1, CB2, and CB1/2(TMIII)

Cannabinoid LigandCB1 Wild TypeKi or KdCB2 Wild TypeKi or KdCB1/2(TMIII) ChimeraKi or Kd
nM
CP 55,9403.8  ± 0.84.2  ± 0.810.7  ± 1.5
WIN 55,212-221.7  ± 6.92.3  ± 0.24.8  ± 0.5
JWH 0155200  ± 190013.81-a 1000  ± 300
(383)1-a
JWH 0189.8  ± 2.83.1  ± 0.31.4  ± 0.2
(R)-Methanandamide35.1  ± 0.28151-b 21.8  ± 5.4
Indomethacin morpholinamide>20004351-c >2000
SR 141716A5.9  ± 0.7>10001-d 11.8  ± 1.0

Binding constants are presented as mean ± S.E. values from at least two experiments performed in duplicate.

  • ↵1-a Ki values from Showalter et al. (1996).

  • ↵1-b Ki value from Khanolkar et al. (1996).

  • ↵1-c Ki value from Gallant et al. (1996).

  • ↵1-d Ki value from Shire et al. (1996).