Table 1

Assays for individual P-450s

P-450^1-a	Substrate	Concentration	mAb^1-b	P-450 Concentration	Product	Internal Standard^1-c	References
		μM	μl/ml	nM
3A4	Testosterone	200	10	15	6β-OH-Testosterone	6β-OH-Progesterone	Hanioka et al. (1990)
3A5	Testosterone	200	10	15	6β-OH-Testosterone	6β-OH-Progesterone	Hanioka et al. (1990)
3A7	Testosterone	200	10	15	6β-OH-Testosterone	6β-OH-Progesterone	Hanioka et al. (1990)
1A1	Phenanthrene	300	10	25	9,10-Dihydrodiol	B[a]P 9,10-Diol	Shou et al. (1994)
1A2	Estrone	100	10	50	2-OH-Estrone	B[a]Pcis-4,5-Diol	Shou et al. (1997)
2A6	Coumarin	100	10	20	7-OH-Coumarin	7-Ethoxycoumarin	Yun et al. (1991)
2B6	Diazepam	200	10	50	Nordiazepam	2-oxo-Quanzepam	Yang et al. (1998)
2C8	Taxol	25	10	25	6α-OH-Taxol	Baccatin III	Rahman et al. (1994)
2C9	Flurbiprofen	20	10	15	4′-OH-Flurbiprofen	B[a]A 5,6-Diol	Tracy et al. (1995)
2C19	Mephenytoin	100	10	25	4′-OH-Mephenytoin	Nirvanol	Goldstein et al. (1994)
2D6	Bufuralol	25	10	10	1′-OH-Bufuralol	Temazepam	Crespi et al. (1995)
2E1	Chlorzoxazone	200	10	50	6-OH-Chlorzoxazone	B[a]A 5,6-Diol	Peter et al. (1990)

↵1-a Baculovirus-expressed individual P-450s were coexpressed with OR in Sf21 insect cells as described in the text.
↵1-b mAb concentration was expressed as μl of mouse ascites pml incubation mixture.
↵1-c Internal standard was used for metabolite quantitation.