Table 2

Cl and Na+ fluxes (J), and apparent permeability coefficient (Papp) of d-mannitol across the pigmented rabbit conjunctiva in the absence and presence of 10 μM UTP added mucosally

ClfluxNa+fluxd-Mannitol Papp
Control10 μM UTPControl10 μM UTPControl10 μM UTP
μEq/cm2/h μEq/cm2/h ×10−7cm/s
Js m 0.59  ± 0.05 (10)0.83  ± 0.04 (10)2-a 0.26  ± 0.04 (7)0.32  ± 0.05 (7)1.82  ± 0.20 (10)2.47  ± 0.32 (10)
Jm s 0.34  ± 0.05 (7)0.41  ± 0.04 (7)0.40  ± 0.05 (7)0.45  ± 0.06 (7)2.10  ± 0.29 (7)2.57  ± 0.37 (7)
Jnet 0.25  ± 0.062-b 0.42  ± 0.052-b −0.14  ± 0.052-b −0.13  ± 0.062-b −0.28  ± 0.11−0.11  ± 0.16
ΔJnet 0.17 ± 0.01−0.01 ± 0.080.18 ± 0.18
ΔJIsc,eq 0.25 ± 0.02

Data represent mean ± S.E. Numbers in parentheses are number of tissues used. Transport of 36Cl (0.5 μCi/ml), 22Na (1 μCi/ml), and d-[3H]mannitol (10 μCi/ml) was studied in the mucosal absence and presence of 10 μM UTP under short-circuit condition. Jnet equals Jsm minus Jms. ΔJIsc,eq is net flux based on Isc measurements.

  • 2-a Significantly different from control.

  • 2-b Significantly different from zero.