Table 2

Kinetic parameters for the uptake of DNP-SG into CMVs

VmaxKmVmax/Km
nmol/min/mg protein μM μl/min/mg protein
Mouse0.38  ± 0.0315.0  ± 3.525.5
Rat1.90  ± 0.0929.6  ± 1.964.2
Guinea pig0.15  ± 0.0116.1  ± 1.29.4
Rabbit0.47  ± 0.0655.8  ± 10.98.4
Dog0.23  ± 0.0230.0  ± 3.77.7
Human2-a 0.701  ± 0.262193  ± 203.8

Initial velocity of the uptake of DNP-SG was fitted to the Michaelis-Menten equation using nonlinear least-squares program. Results are mean ± computer-calculated S.D.

• 2-a Taken from a previous report (mean ± S.E.,n = 3) (Niinuma et al., 1999).