Kinetic parameters for the uptake of DNP-SG into CMVs
| Vmax | Km | Vmax/Km | |
|---|---|---|---|
| nmol/min/mg protein | μM | μl/min/mg protein | |
| Mouse | 0.38 ± 0.03 | 15.0 ± 3.5 | 25.5 |
| Rat | 1.90 ± 0.09 | 29.6 ± 1.9 | 64.2 |
| Guinea pig | 0.15 ± 0.01 | 16.1 ± 1.2 | 9.4 |
| Rabbit | 0.47 ± 0.06 | 55.8 ± 10.9 | 8.4 |
| Dog | 0.23 ± 0.02 | 30.0 ± 3.7 | 7.7 |
| Human2-a | 0.701 ± 0.262 | 193 ± 20 | 3.8 |
Initial velocity of the uptake of DNP-SG was fitted to the Michaelis-Menten equation using nonlinear least-squares program. Results are mean ± computer-calculated S.D.
↵2-a Taken from a previous report (mean ± S.E.,n = 3) (Niinuma et al., 1999).