Quantitation of DNA-PK components coimmunoprecipitated with anti-DNA-PKcs Ab and anti-Ku 70 Ab (Fig. 5)
| APA-SG | ||||
|---|---|---|---|---|
| 0 mM | 1 mM | 3 mM | 5 mM | |
| DNA-PKcs Ab | ||||
| DNA-PKcs | 100 | 178 | 163 | 142 |
| Ku70 | 100 | 35 | 12 | 15 |
| Ku80 | 100 | 80 | 0 | 0 |
| KU70 Ab | ||||
| DNA-PKcs | 100 | 25 | 20 | 8 |
| Ku70 | 100 | 96 | 100 | 97 |
| Ku80 | 100 | 45 | 20 | 34 |
Quantitation was achieved using NIH Image software. Absorbance values in the absence of drug were designated as 100% (column 1). For each row, other data were calculated based on this 100% value.