Assay | Protein Preparation | Activities | Activity4-a | |
---|---|---|---|---|
+0 μM testosterone | +100 μM testosterone | |||
pmol product formed/min/pmol P-450 | % | |||
Bufuralol 1′-hydroxylase | CYP2D6 | 8.38 ± 0.21 | 7.69 ± 0.26 | 92 |
Human liver microsomes | 0.13 ± 0.01 | 0.08 ± 0.014-b | 62 | |
Coumarin 7-hydroxylase | CYP2A6 | 2.26 ± 0.11 | 2.26 ± 0.09 | 100 |
Human liver microsomes | 0.41 ± 0.09 | 0.33 ± 0.034-b | 80 | |
EROD | CYP1A2 | 5.86 ± 0.79 | 5.87 ± 0.63 | 100 |
Human liver microsomes | 0.23 ± 0.06 | 0.16 ± 0.044-b | 70 |
Assays were performed as described in Experimental Procedures with bacterial membranes containing 10 pmol of recombinant P-450 isozymes with CPR or with human liver microsomes corresponding to 200 pmol P-450.