Treatment | Immunoreactive CYP Protein | Erythromycin Demethylation | |||||
---|---|---|---|---|---|---|---|
1A1 | 1A2 | 2B1/2 | 2C11 | 2D1 | 3A | ||
% of control | nmol/min/mg protein | ||||||
Control | 100 ± 9 | 100 ± 17 | 100 ± 15 | 100 ± 18 | 100 ± 5 | 100 ± 8 | 0.17 ± 0.03 |
IL-2 | 63 ± 181-a | 75 ± 8 | 62 ± 91-a | 64 ± 121-a | 61 ± 101-a | 62 ± 91-a | 0.10 ± 0.021-a |
Retinoic acid | 102 ± 45 | 133 ± 33 | 75 ± 12 | 76 ± 24 | 122 ± 21 | 77 ± 12 | 0.14 ± 0.03 |
Retinoic acid + IL-2 | 109 ± 36 | 141 ± 29 | 87 ± 6 | 74 ± 23 | 105 ± 9 | 85 ± 8 | 0.16 ± 0.03 |
n-Butyric acid | 91 ± 17 | 101 ± 16 | 88 ± 7 | 71 ± 8 | 89 ± 6 | 78 ± 16 | 0.16 ± 0.02 |
n-Butyric acid + IL-2 | 99 ± 8 | 130 ± 42 | 81 ± 6 | 82 ± 9 | 94 ± 10 | 86 ± 13 | 0.14 ± 0.02 |
DMSO | 108 ± 34 | 96 ± 40 | 90 ± 9 | 88 ± 10 | 78 ± 9 | 79 ± 15 | 0.13 ± 0.02 |
DMSO + IL-2 | 92 ± 27 | 83 ± 39 | 90 ± 18 | 99 ± 8 | 95 ± 12 | 84 ± 10 | 0.15 ± 0.03 |
After cell attachment, hepatocytes were cultured for 24 h with or without IL-2 (350 U/ml), retinoic acid (10 μM), n-butyric acid (3 mM), and DMSO (2%). Microsomal proteins (10 μg) were separated by SDS-10% PAGE, transferred to nitrocellulose sheets, revealed by antibodies, quantified by laser densitometry, and expressed as percentage of that in control cells. Erythromycin demethylation was measured in cell homogenates. Results are mean ± S.E.M. for four or five cultures.
↵1-a Different from the corresponding incubation without IL-2 (P < .05).