Table 1

Intraday variability in 1′-OH MDZ formation and distribution in Caco-2 cells

CultureAmount of 1′-OH MDZ Formed1-aA/B Ratio
ApicalBasolateralCellularTotal
pmol
1111.846.029.5157.82.43
2139.149.327.9188.42.82
3128.343.624.2171.82.95
4154.858.523.5213.32.65
5124.550.920.4175.42.44
6116.346.523.3162.92.50
Mean129.149.124.8178.32.63
SD15.85.23.320.20.22
CV12.2%10.7%13.411.3%8.2%
  • 1-a Caco-2 monolayers were incubated with 4.5 nmol apically applied MDZ for 30 min at 37°C.