Characteristics of temocaprilat uptake into isolated hepatocytes (mean ± S.E., N = 3)
| Treatment | Uptake (% of Control) |
|---|---|
| Rotenone (30 μM) | 22.9 ± 2.6 |
| FCCP (2 μM) | 28.7 ± 4.7 |
| PCMBS (100 μM) | 15.7 ± 1.7 |
| DIDS (100 μM) | 14.7 ± 3.2 |
| 4°C | 4.3 ± 0.0 |
| Na+→Li+ | 108.2 ± 1.5 |
The uptake of [3H]temocaprilat (0.1 μM) by the isolated hepatocytes was studied in the presence of metabolic inhibitor, sulfhydryl-modifying reagent and anion exchange inhibitor. These drugs were added to the cell suspension 5 min before the addition of [3H]temocaprilat. Effect of incubation temperature and Na+ was also examined.