Table 2

Apparent kinetic parameters for metabolism of substrates for human B-lymphoblastoid cell expressed CYP2B6

SubstrateKm (μM)Vmax(pmol/min/mg Protein)Autoactivationn2-a
3-Cyano-7-ethoxycoumarin71.3  ± 22.92-b 6.9  ± 1.3NoND2-c
4-Chloromethyl-7-ethoxycoumarin33.7  ± 4.311.4  ± 0.8NoND
Benzyloxyresorufin1.3  ± 0.18.7  ± 0.4NoND
Benzphetamine93.4  ± 0.50.49  ± 0.03NoND
Midazolam46.1  ± 4.57.8  ± 0.4NoND
Testosterone50.5  ± 4.887.0  ± 2.9Yes1.3 ± 0.1

  • ↵2-a Number of sites bound by activator.

  • ↵2-b Parameter estimate ± S.E. of the parameter estimate.

  • ↵2-c Not determined.