Table 4

Effect of amphetamine on midbrain TH protein levels

TreatmentTime after amphetamineMidbrain TH protein
days μg/mg of cellular protein
Saline10.43  ± 0.14
Amphetamine10.49  ± 0.12
Saline30.29  ± 0.03
Amphetamine30.26  ± 0.08
Saline140.34  ± 0.10
Amphetamine140.26  ± 0.06
months
Saline40.47  ± 0.14
Amphetamine40.35  ± 0.10

Rats were administered 3 mg/kg amphetamine four times once every 2 hr, and midbrains were removed at the times. Midbrain TH protein was measured using Western blot analysis and normalized to the micrograms of total cellular protein subjected to electrophoresis.

  • Data represent the mean ± S.E. from 5 or 6 rats.

  • No significant differences were calculated by ANOVA (P > .05) between values.