Table 1

Effect of amphetamine treatment on striatal dopamine and TH protein levels

TreatmentTime after amphetamineStriatal dopamineStriatal TH protein
days pmol/mg of tissue μg/mg of cellular protein
Saline191  ± 4N.D.
Amphetamine124  ± 41-a N.D.
Saline393  ± 3N.D.
Amphetamine315  ± 31-a N.D.
Saline1484  ± 42.1  ± 0.2
Amphetamine1418  ± 31-a 0.8  ± 0.11-a
months
Saline4106  ± 41.7  ± 0.1
Amphetamine447  ± 3a,b 0.8  ± 0.11-a

Rats were administered saline or 3 mg/kg amphetamine four times once every 2 hr and killed at the designated times. Striatal dopamine levels were determined by HPLC, and striatal TH protein levels were determined by Western blot analysis. Data represent the mean ± S.E. from 6 to 8 rats.

  • 1-a P < .001 compared with time-matched saline-treated rats.

  • 1-b P < .01 compared with amphetamine-treated rats at 1, 3 or 14 days.