Control | + Gpp(NH)p | Ratio ofKi Values | |||
---|---|---|---|---|---|
Ki(nM) | η | Ki (nM) | η | ||
DAMGO | 19.7 ± 5.2 | 0.48 ± 0.05 | 68.2 ± 12.4 | 0.92 ± 0.03 | 3.5 |
NalBzoH | 2.8 ± 0.8 | 0.86 ± 0.01 | 0.9 ± 0.5 | 0.91 ± 0.03 | 0.32 |
Diprenorphine | 1.4 ± 0.8 | 0.89 ± 0.1 | 0.44 ± 0.1 | 0.83 ± 0.1 | 0.31 |
CHO/MOR-1 cell membranes were prepared and competitions of the indicated ligands were performed with 3H-diprenorphine (0.5 nM) with and without Gpp(NH)p (100 μM). Results are the means ± S.E.M. of three independent determinations.