Table 1

Analysis of individual behavioral components of PTZ-induced behavioral changes as a function of drug treatment

“Saline-like” Features”Vehicle + VehicleVehicle + PTZGanaxolone + PTZDiazepam + PTZ
  1 Walking22.75  ± 1.75*1.25  ± 0.6320.25  ± 1.50*1.75  ± 0.25
  2 Rearing23.00  ± 0.41*0.50  ± 0.5019.75  ± 1.79*0.25  ± 0.25
  3 Grooming21.75  ± 2.13*0.50  ± 0.5010.00  ± 0.71*0.75  ± 0.48
  4 Sleeping0.00  ± 0.000.00  ± 0.000.25  ± 0.250.00  ± 0.00
  5 Tail curled1.75  ± 0.860.50  ± 0.501.50  ± 0.650.00  ± 0.00
“PTZ-like” Features
  6 Motionlessness0.00  ± 0.00*25.75  ± 2.34.00  ± 0.82*26.75  ± 1.55
  7 Hindlimbs visible0.00  ± 0.00*12.25  ± 1.551.25  ± 0.63*6.00  ± 0.41*
  8 Hiccups0.00  ± 0.001.75  ± 1.111.25  ± 0.481.75  ± 0.25
  9 Tail straight0.00  ± 0.00*12.25  ± 3.120.75  ± 0.48*9.25  ± 2.10*
 10 Nose to corner0.00  ± 0.00*3.75  ± 1.030.00  ± 0.00*7.25  ± 1.25
 11 Tail along wall0.00  ± 0.00*11.50  ± 1.712.00  ± 0.92*6.25  ± 1.65*

The numbers of animals (mean ± S.E.M.) that displayed specific behaviors in one of the four observational periods were summed to yield a total theoretical maximal score of 32. Behaviors 1 to 5 represent “saline-like” behaviors, and behaviors 6 to 11 represent “PTZ-like” behaviors. Ganaxolone and diazepam were given as pretreatments 15 min and 30 min, respectively, before the administration of 45 mg/kg PTZ. Drugs were administered in doses equivalent to their ED50 values for protection against convulsions engendered by 70 mg/kg PTZ. * indicates a significant difference (P < .05; Dunnett’s one-tailed test) from PTZ alone.