Source of vesicles | Substrate | S-8921 (μM) | Substrate Accumulation (nmol/mg protein) |
---|---|---|---|
Ileum | TCA | None | 0.546 ± 0.025 |
Ileum | TCA | 1 | 0.409 ± 0.032** |
Ileum | TCA | 10 | 0.138 ± 0.017** |
Ileum | TCA | 100 | 0.009 ± 0.005** |
Jejunum | d-Glucose | None | 0.319 ± 0.015 |
Jejunum | d-Glucose | 100 | 0.302 ± 0.005 |
Jejunum | l-Alanine | None | 0.010 ± 0.000 |
Jejunum | l-Alanine | 100 | 0.010 ± 0.001 |
Ileum | l-Alanine | None | 0.011 ± 0.000 |
Ileum | l-Alanine | 100 | 0.013 ± 0.001 |
Uptake was initiated at zero time by addition of a medium (70 μl) containing 100 mM mannitol, 150 mM NaCl, 0.05 mM [14C]TCA and 20 mM HEPES/Tris buffer (pH 7.5), or containing 100 mM mannitol, 0.1 mM MgSO4, 100 mM NaSCN, 1 mM HEPES/Tris buffer (pH 7.5) and 22.9 μM d-[14C]glucose or 0.008 μMl-[14C]alanine to vesicles (50 μl). The amount of substrate accumulated in 15 sec is shown and expressed as the mean ± S.D. of three experiments.
** P < .01, statistically significant difference from control group by Dunnett’s multiple comparison test.