Effects of AF-DX 116, 4-DAMP mustard treatment, pertussis toxin treatment and their combination on the contractile response to oxotremorine-M under standard conditions
| Conditions | pEC502-a | |
|---|---|---|
| Control | AF-DX 116 (1 μM) | |
| Control | 7.32 ± 0.13 (8) | 7.22 ± 0.112-b (4) |
| Pertussis toxin | 6.99 ± 0.28 (6) | N.D. |
| 4-DAMP mustard (1 hr) | 5.97 ± 0.302-c (4) | N.D. |
| 4-DAMP mustard (2 hr) | 6.02 ± 0.26 (8) | 5.35 ± 0.082-d (5) |
| 4-DAMP mustard (2 hr) + pertussis toxin | 5.07 ± 0.73 (6) | N.D. |
↵2-a Each pEC50 value represents the mean ± S.E.M. of the experiments conducted. The number of experiments is given in parentheses.
↵2-b The control EC50 value was estimated from the pooled data from eight experiments. However, AF-DX 116 was investigated in only four of these experiments. The control EC50 value for these four experiments was 0.0273 μM (pEC50 = 7.56).
↵2-c The control EC50 value was estimated from the pooled data from eight experiments. However, contractions in 1-hr 4-DAMP mustard-treated colon were investigated in only four of these experiments. The control EC50 value for these four experiments was 0.0380 μM (pEC50 = 7.42).
↵2-d The control EC50 value was estimated from the pooled data from eight experiments. However, the effect of AF-DX 116 on contractions in 2-hr 4-DAMP mustard-treated colon was investigated in only five of these experiments. The control EC50 value for these five experiments was 1.04 μM (pEC50 = 5.98).