Table 2

Saturation binding analysis of cannabinoid receptor-stimulated [35S]GTPγS binding in membranes from various rat brain regions

RegionApparent KDApparentBmax
nM pmol/mg
Frontal cortex3.45  ± 0.4610.5  ± 0.73bc
Cerebellum2.49  ± 0.2613.1  ± 1.27
Hippocampus2.73  ± 0.1912.1  ± 1.00bc
Striatum2.26  ± 0.6214.4  ± 0.75ab
Sensomotor cortex3.00  ± 1.027.5  ± 1.49c
Thalamus2.09  ± 0.258.4  ± 0.83c
Colliculi3.10  ± 0.1610.2  ± 1.52bc
Brainstem3.08  ± 0.879.6  ± 0.90bc
Amygdala2.69  ± 0.5514.7  ± 1.89ab
Hypothalamus2.98  ± 0.6818.5  ± 0.48a

Net WIN 55212-2-stimulated [35S]GTPγS (cannabinoid receptor-activated G-protein) binding was determined as described under “Materials and Methods.” The data represent mean values ± S.E.M. from saturation binding analyses which were conducted in triplicate three or more times in membranes from each region of rat brain. By the Tukey-Kramer HSD test at P < .05, values within a given column that are not significantly different are denoted by the same single letter. For the column of apparent K Dvalues, none of the values are significantly different.