Protein content and β-glucuronidase activity in peritoneal lavage fluids after MSU crystal injection
| Time1-a(hr) | β-Glucuronidase Activity1-b | Protein1-c (mg/ml) | |
|---|---|---|---|
| (U/106cells) | (U/ml) | ||
| 0 | 74 ± 11 (6) | 251 ± 25 (6) | 0.5 ± 0.1 (6) |
| 2 | 4254 ± 2731-d (6) | 1718 ± 1701-d (6) | 5.0 ± 0.31-d (6) |
| 6 | 98 ± 11 (6) | 251 ± 29 (6) | 2.1 ± 0.21-d (6) |
| 16 | 71 ± 7 (6) | 209 ± 26 (6) | 0.7 ± 0.1 (6) |
| 24 | 54 ± 8 (6) | 163 ± 34 (6) | 0.6 ± 0.3 (6) |
↵1-a Mice were treated with MSU crystals (3 mg i.p.) at time 0 and lavage fluids were collected at the reported time-points. Untreated mice (time 0 group) were used for comparative purposes.
↵1-b β-Glucuronidase activity was measured in cell-free lavage fluids as described in “Materials and Methods.” Values are mean ± S.E.M. of (n) per group.
↵1-c Protein concentration in cell-free lavage fluids was quantified using the blue Coomassie reaction as described in “Materials and Methods.” Values are mean ± S.E.M. of (n) per group.
↵1-d P < .05 vs. untreated mice (time 0).