Table 1

Liver history (age and gender) and values used in comparing CYP2A6 immunoactivity with nicotine metabolism

LiverAgeSexCYP2A6 immunoactivity1-aCoumarin inhibition of cotinine formation1-bKm VmaxRatio of Vmax over Km
yr nmol/mg of protein/hr1-b μM nmol/mg of protein/hr
K1227FUnknownUnknown136.60.508
K2050F1.33610.2647828.50.365
K2755F2.0809.3686428.40.444
L1853F2.1508.603228.20.373
L2047F0.74511.8295216.80.323
L243F5.10042.0965068.01.360
L3136F3.81115.0483822.40.589
L328F0.0001.2431064.20.040
L3630F5.61050.7446096.11.602
L3816F4.62049.0455698.31.755
L3919F1.94819.6435225.20.485
L4436F2.01716.6537334.20.468
L455F1.49816.7126826.80.394
L6016F10.52099.02155120.02.182
L6146F0.0425.0961319.10.070
L6255F0.1764.595698.50.123
L6342F0.7526.7236616.50.250
L196M1.67814.8914623.60.513
L2513M0.6465.9476010.40.173
L2631M1.66710.2646815.80.232
L2717M0.0342.3331627.50.046
L299M2.40710.0244518.20.404
L3023M0.2403.912516.50.127
L332M0.2392.4431238.40.069
L3428M3.20316.0533022.20.740
L4049M2.68719.6296034.80.580
L414M0.55512.8459124.80.273
L4342M1.79922.5364234.80.829
L4743M1.0286.741217.80.373
L238Unknown0.98911.0535617.30.309
L644Unknown0.77120.34710422.60.217

  • ↵1-a Values were calculated from band densities obtained from Western blots.

  • ↵1-b Amount of cotinine formation inhibited by the addition of 150 μM coumarin.