Kinetic parameters for debrisoquine 4-hydroxylase and bunitrolol 4-hydroxylase activities in liver microsomes of male mice and rats
| Substrate | Species/Strain | Km(μM) | Vmax (pmol/min/mg protein) |
|---|---|---|---|
| Debrisoquine | Mouse/C57BL/6 | 929 ± 66 | 114 ± 4 |
| Mouse/ddY | 796 ± 64 | 55 ± 4 | |
| Rat/Wistar | 18.6 ± 1.1 | 118 ± 9 | |
| 464 ± 70 | 728 ± 48 | ||
| Bunitrolol | Mouse/C57BL/6 | 13.1 ± 1.9 | 1530 ± 190 |
| Mouse/ddY | 13.7 ± 2.8 | 1140 ± 200 | |
| Rat/Wistar1-a | 1.36 ± 0.21 | 2730 ± 140 |
Each value represents the mean ± S.E. of three determinations.
↵1-a Parameters for bunitrolol 4-hydroxylation in rats were quoted from Suzuki et al. (1991).