Table 2

Effects of TCA, organic anions, organic cations and ouabain on the uptake of GPFX (5 μM) by isolated rat hepatocytes

Anions or Neutal (μM)% of Control (PSinf)Cations (μM)% of Control (PSinf)
Control100.0  ± 4.1Control100.0  ± 2.9
TCA 2091.2  ± 12.9Cimetidine 3093.5  ± 2.7
10091.0  ± 8.210091.8  ± 2.1
50092.5  ± 7.430085.4  ± 2.0
DBSP 596.5  ± 4.2PAEB 2097.7  ± 1.8
2093.3  ± 3.7200110.9  ± 10.2
10099.2  ± 7.0 d-Tubocurarine 2096.7  ± 2.7
ICG 592.1  ± 2.320098.0  ± 4.4
2077.9  ± 12.2Quinidine 385.7  ± 3.5
10090.2  ± 18.53079.7  ± 2.9
Pravastatin 2087.2  ± 12.010063.9  ± 4.72-a
100100.1  ± 7.8Verapamil 393.6  ± 4.8
50081.3  ± 4.93084.2  ± 5.0
Ouabain 2090.6  ± 5.610072.9  ± 5.02-a
20089.6  ± 4.4Vincristine 2086.6  ± 6.5
50088.3  ± 4.320077.3  ± 7.72-b
50056.0  ± 8.02-a
  • Uptake of [14C]-GPFX was measured with or without TCA, organic anion, cations or TCA. Each bar represents the mean ± S.E. six to nine determinations in two to three different preparations.

  • 2-a P < .01.

  • 2-b P < .05, (significantly different from controls using Dunnett’s test).