Table 1

Summary of the calculated unidirectional transfer constants (K in) and initial volumes of distribution (V i) for [3H]AZT, [3H]thymidine and d-[14C]mannitol into the CNS determined afterin situ brain perfusion in the guinea pig

IsotopeCerebrumCerebellumCSF
KinViKinViKin
μl/min/g ml/100 g μl/min/g ml/100 g μl/min/g
d-[14C]Mannitol0.23  ± 0.04a 0.52  ± 0.080.31  ± 0.12a 0.65  ± 0.23
(n = 15–21a; n = 51-b)0.32  ± 0.071-b
[3H]AZT0.83  ± 0.10a 1.63  ± 0.200.66  ± 0.08a 1.80  ± 0.14
(n = 15–21a; n = 51-b)0.95  ± 0.121-b 0.72  ± 0.151-b 0.96  ± 0.091-b
[3H]Thymidine1-c 2.56  ± 0.21a 1.68  ± 0.442.68  ± 0.41a 1.50  ± 0.87
(n = 27–46a; n = 61-b)2.76  ± 0.311-b 2.62  ± 0.361-b 2.67  ± 0.321-b
  • Values ± S.E.M. have been determined from multiple-time point analysisa and single-time point analysisb at a bilateral brain perfusion time of 30 min.

  • 1-b Values have been corrected ford-[14C]mannitol/plasma space.

  • 1-c Values are for comparison (Thomas and Segal, 1996).