Drug application and related inhibition
| Drug | Application1-a | Inhibition | Change1-b |
|---|---|---|---|
| % | |||
| GABA young | 86.2 ± 12 nA/sec | 29.9 ± 1.4 | |
| GABA old | 96.0 ± 10 nA/sec | 30.9 ± 1.1 | |
| ISO old | 40.7 ± 5.4 nA | 36.3 ± 4.9 | 4.6 ± 5.1 |
| FORSK young (100 μM) | 5.4 ± 1.1 psi | 42.3 ± 5.2 | 12.2 ± 5.7 |
| FORSK old (100 μM) | 9.9 ± 1.8 psi1-c | 29.6 ± 3.1 | −6.2 ± 3.6 |
| 7β-FORSK young (100 μM) | 4.9 ± 1.8 psi | 55.2 ± 6.1 | 26.5 ± 7.5 |
| 7β-FORSK old (1 mM) | 5.5 ± 1.1 psi | 33.0 ± 3.7 | 2.08 ± 3.3 |
| 100 μM Sp-cAMPS young | 4.9 ± 1.6 psi | 53.3 ± 3.1 | 26.0 ± 2.8 |
| 10 μM Sp-cAMPS old | 1.4 ± 0.4 psi | 32.5 ± 2.7 | 3.2 ± 1.8 |
| 100 μM Sp-CAMPS old | 5.24 ± 1.0 psi | 24.8 ± 2.9 | −3.9 ± 2.7 |
| 1 mM Sp-cAMPS old | 6.6 ± 1.5 psi | 50.3 ± 3.6 | 15.2 ± 3.8 |
Values are mean ± S.E.M.
↵1-a Drug application from the electrode in nanoamps or psi. When applications times are <120 secs, then time influences the amount of drug being ejected. If time is not noted, this indicates application times of >120 secs, and at these times, a steady state level is reached and the pressure or current adequately represents the “dose.”
↵1-b Percent change indicates the difference in percent inhibition to GABA before drug application and during drug application. For example, if the GABA-induced inhibition before FORSK was 20% and the inhibition during FORSK was 70%, then the percent change would be 50%.
↵1-c P < .05 vs. young FORSK application.