Table 2

Acetaldehyde oxidation activity of purified rat P450s

P450Acetate Formation
nmol/min/nmol P450
1A14.5
1A212.5
2B12.8
2C117.8
2E137.6
3A20.5
4A211.2
2D12.5

The reaction conditions were the same as those described in table 1, except for the use of a reconstituted system containing purified P450 (30 pmol), NADPH-P450 reductase (0.3 U) and DLPC (5 μg) being used instead of microsomes.