Table 3

Correlation of cAMP accumulation and relaxant efficacy of maximal concentrations of various receptor agonists

Agonist (concentration)Fold Stimulation of cAMP Accumulation3-aRelaxation (Rank)3-b
%
Forskolin (10 μM)14.93  ± 3.14103.4  ± 0.72 (1)
Isoproterenol (1 μM)2.51  ± 0.2597.5  ± 5.93 (2)
Cicaprost (0.8 μM)2.45  ± 0.2543.9  ± 5.43 (3)
PGE2 (10 μM)2.28  ± 0.28NA3-c
PGE1 (10 μM)2.27  ± 0.15NA
PGI2 (10 μM)1.52  ± 0.12NA
5-HT (10 μM)1.43  ± 0.20NA
Dopamine (10 μM)1.42  ± 0.2026.7  ± 3.95 (5)
VIP (10 μM)1.33  ± 0.1622.4  ± 2.77 (7)
Dimaprit (10 μM)1.32  ± 0.12NA
5-MT (10 μM)1.25  ± 0.05NA
CGS-21680 (10 μM)1.12  ± 0.0324.5  ± 3.82 (6)
2-Chloroadenosine (10 μM)1.09  ± 0.0713.9  ± 4.46 (8)
SKF-38393 (10 μM)1.08  ± 0.0530.2  ± 0.90 (4)
PGD2 3-d (10 μM)1.08  ± 0.06NA
Vasopressin (10 μM)0.97  ± 0NA
Secretin (10 μM)0.93  ± 0.03NA
  • 3-a Fold stimulation over basal, mean ± S.E.M. of 3 to 12 experiments.

  • 3-b Percent relaxation of histamine-induced contraction, mean ± S.E.M. of 3 to 8 experiments.

  • 3-c NA, not applicable.

  • 3-d PGD2, prostaglandin D2.