TABLE 1

IC₅₀ values for AM211 in the in vitro assays

All IC₅₀ values are the mean ± S.D. with n values indicated in parentheses.

Receptor	IC₅₀
	nM
[³H]PGD₂ radioligand membrane binding
Human DP2	4.9 ± 1.3 (6)
Mouse DP2	7.8 ± 2.2 (4)
Guinea pig DP2	4.9 ± 1.9 (4)
Rat DP2	10.4 ± 2.8 (3)
Human DP2 + human SA^{^a}	12.2 ± 3.4 (6)
Mouse DP2 + mouse SA^{^a}	20.1 ± 8.8 (5)
Guinea pig DP2 + guinea pig SA^{^a}	22.9 ± 6.0 (4)
Rat DP2 + rat SA^{^a}	34.2 ± 13.8 (3)
Human DP2 [³⁵S]GTPγS binding	13.3 ± 3.7 (6)
In vitro functional assays
Human whole blood ESC	2.7 ± 0.9 (7)
Guinea pig whole blood ESC	21.5 (2)^{^b}
Human basophil CD203c	2.5 (2)^{^b}

↵a Potency determined in the presence of 0.2% species-specific serum albumin (SA).
↵b S.D. not calculated for studies involving n values of <3.