TABLE 4

Transport of fluorescent bile acid derivatives by uptake transporters

Transport experiments were performed as described in Material and Methods. The substrate conc. were 5 µM, and transport was measured for 5 minutes. Uptake values were corrected for uptake into WT CHO cells. Results are given in picomole per milligram protein × 5 minutes. Data are mean ± S.D. from two to four independent experiments with three technical replicates.

CompoundNTCPOATP1B1OATP1B3OATP2B1ASBT
CenNBDNT67.0 ± 30.0NT26.1 ± 13.7NT
CenDNS20.3 ± 10.0NT1577 ± 509175 ± 20.2NT
CenPBNT21.6 ± 13.1306 ± 188NTNT
CDCenNBDNT908 ± 3098545 ± 32001779 ± 405NT
CDCenDNSNTNT1524 ± 311141 ± 26.61NT
CDCenPBNTNT162 ± 59.760.5 ± 18.4NT
AcenNBDNTNTNTNTNT
AcenDNSNTNTNTNTNT
AcenPBNDNTNTNTND
  • ND, not determined; NT, no transport.