TABLE 2

Pharmacokinetic parameters of blebbistatin, NBleb, and AmBleb from experiments using rat and human hepatocytes

Cl_int = (ln2/t_1/2) × ((cell number/tissue weight)/(cell number/incubation volume)) × (liver weight/body weight), where t_1/2 is the time required to achieve 50% of the initial concentration at t = 0 min. Cl_H = (Cl_int × fu × HPF)/(Cl_int × fu + HPF), where HPF = Q_H × plasma/blood ratio, where Q_H = 55.2 ml/min per kilogram for rat, 20.7 ml/min per kilogram for human, plasma/blood ratio = 0.63 for rat, 0.57 for human. E_H = Cl_H/Q_H. F (bioavailability) = 100*(1−E_H).